RNAscope common questions, troubleshooting, and tips and tricks
When performing RNAscope image analysis, you can set yourself up for success with a combination of thoughtful experimental design, rigorous assay execution and validation. At Indica Labs, the most common challenges we see in RNAscope image analysis include chromogenic staining that has saturated to black, which causes color deconvolution challenges, loss of nuclear morphology due to over-digestion of tissue, and failing to verify and validate an image analysis solution before scaling up.
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